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What is SPR?
Surface Plasmon Resonance (SPR) is a physical phenomenon that occurs on the surface of a metal film when plane-polarized light is fully reflected. When the material in the vicinity of the gold film changes, the resonance angle of the incident light (SPR angle) will also change simultaneously. By recording the resonance angle in real time, the detector can obtain the dynamic process of interaction between biomolecules in the microfluidic channel laminated to the gold film.
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SPR Advantages
SPR Sensorgram
图文展示
PHASE | MEANING |
Baseline | The initial signal when the buffer solution flows through the chip before the test begins. |
Binding | The process in which analytes are primarily bound to the ligand. |
Equilibrium | The stage at which the binding and dissociation of analytes on the ligand reach equilibrium. |
Dissociation | The process by which the buffer solution removes bound analytes from the ligand. |
Regeneration | The process of flushing the chip surface to remove any remaining analytes at the end of an experiment, ensuring the ligand remains intact for subsequent analysis. |